Ethanolic Extract of Moringa’s leaves (Moringa oleifera) Induce Senescence on Adenocarcinomic Alveolar Basal Epithelial Cells (A549 Cell-lines)
DOI:
https://doi.org/10.47701/icohetech.v1i1.791Keywords:
Anticancer, ethanolic extract of Moringa, , in vitro, senescence, viabilityAbstract
Moringa (Moringa oleifera) is a plant that widely used as traditional medicines. The use of Moringa as herbal medicine until now is widely studied due to its high antioxidant activity and its role as an anticancer in vitro. This study aims to determine the effect of the ethanolic extract of Moringa's leaf on the viability and senescence of lung cancer cells (A549 cell-lines). Moringa leaves were extracted with 70% ethanol solvent and tested for cell viability by MTS test. Senescence detection test were conducted by Senescence Associated-β-Galactosidase (SA-β-Gal) staining. The result of the viability test showed that the ethanolic extract of Moringa leaves was able to reduce the viability of cancer cells by 1000 µg / ml of IC50 value. The senescence detection test results used SA-β-Gal showed that the ethanolic extract of Moringa leaves was able to induce senescence up to 25% in cancer cells. The same test also performed on normal cells (MCF-12A cell-lines). The result of the assay that conducted on normal cells showed that the ethanolic extract of Moringa leaves conduct greater toxicity in cancer cells, and did not significantly induce senescence in normal cells. These results indicate the potential of Moringa as a cancer therapy agent and could be used as a basis for further research on the potential for Moringa as an anticancer.
References
Ganguly, S. (2013). Indian ayurvedic and traditional medicinal implications of indigenously available plants, herbs and fruits: a review. International Journal of Research in Ayurveda & Pharmacy, 4(4), 623–625.
Abd Rani, N. Z., Husain, K., & Kumolosasi, E. (2018). Moringa genus: a review of phytochemistry and pharmacology. Frontiers in Pharmacology, 9.
Bray, F., Ferlay, J., Soerjomataram, I., Siegel, R. L., Torre, L. A., & Jemal, A. (2018). Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: A Cancer Journal for Clinicians.
Pistritto, G., Trisciuoglio, D., Ceci, C., Garufi, A., & D’Orazi, G. (2016). Apoptosis as anticancer mechanism: function and dysfunction of its modulators and targeted therapeutic strategies. Aging, 8(4), 603–619.
Ramsey, M. R., & Sharpless, N. E. (2006). ROS as a tumour suppressor? Nature Cell Biology, 8(11), 1213–1215.
Hornsby, P. J. (2007). Senescence As an Anticancer Mechanism. Journal of Clinical Oncology, 25(14), 1852–1857
Hayflick, L., & Moorhead, P. S. (1961). The serial cultivation of human diploid cell strains. Experimental Cell Research, 25(3), 585–621.
Hayflick, L. (1965). The limited in vitro lifetime of human diploid cell strains. Experimental Cell Research, 37(3), 614–636.
Collado, M., Blasco, M. A., & Serrano, M. (2007). Cellular senescence in cancer and aging. Cell, 130(2), 223–233.
Jung IL (2014) Soluble Extract from Moringa oleifera Leaves with a New Anticancer Activity. PLoS ONE 9(4): e95492
Koffi E., Sea T., Dodehe Y., Soro S. (2010) Effect of solvent type on extraction of polyphenols from twenty three ivorian plants. J. Anim. Plant Sci. 5:550–558.
Al-Asmari A.K., Albalawi S.M., Athar M.T., Khan A.Q., Al-Shahrani H., & Islam M. (2015). Moringa oleifera as an anti-cancer agent against breast AND colorectal cancer cell lines. PLoS ONE 10(8): e0135814.
Abcam. (2017). MTS cell proliferation assay kit (colorimetric) (AB197010). [Online]. Tersedia: https://www.abcam.com/ps/products/197/ab197010/documents/ab197010%20MTS%20Cell%20Proliferation%20Colorimetric%20Assay%20Kit%20Protocol%20v3%20(website).pdf
Priyandoko, D., Ishii, T., Kaul, S. C., & Wadhwa, R. (2011). Ashwagandha Leaf Derived Withanone Protects Normal Human Cells Against the Toxicity of Methoxyacetic Acid, a Major Industrial Metabolite. PLoS ONE, 6(5), e19552.
Navarro, S. L., Li, F., & Lampe, J. W. (2011). Mechanisms of action of isothiocyanates in cancer chemoprevention: an update. Food & Function, 2(10), 579.
