MICROBIAL SAFETY OF STREET FOOD SAUCES : DETECTION OF ESCHERICHIA COLI AMONG VENDORS IN KLATEN REGENCY
DOI:
https://doi.org/10.47701/n3tpt073Keywords:
processed egg roll sauce, Most Probable Number, Biochemical Test, Gram Staining, Eschericia coliAbstract
Egg rolls are one type of food that requires additional ingredients in the form of sauce. Many egg sellers use bicycles or two-wheeled vehicles, with merchandise placed behind the bicycle or motorbike. However, the cleanliness of egg rolls is often ignored, one of the causes is exposure to dust and air that enters the food. This study aims to test for contamination of Eschericia coli bacteria and identify the presence of these bacteria in the sauce used in egg rolls. The method used in this study was a field survey with random and descriptive sampling techniques to determine the presence or absence of Eschericia coli in several types of sauces sold with egg rolls. Sampling was carried out by selecting 10 types of sauces from egg roll food sold around in Klaten Regency. Tests for Eschericia coli bacteria were carried out at the Biology Laboratory of Sebelas Maret University, Surakarta, and one sample as a negative control using the most probable number (MPN) method, Escherichia coli test and gram staining. The results of the Most Probabe Number test and confirmation test showed that there was Eschericia coli, while from the results of gram staining, the detected bacteria were included in the gram-negative category. The results of the biochemical test showed that the bacteria in the sample included the Enterobacter and Escherichia coli species. The tests carried out showed that all results did not meet the standards set in SNI 01-3546-2004, which regulates the maximum limit of the Total Plate Count (TPC) in tomato sauce is 2 x 10² colonies/g, while according to the Regulation of the Head of the Food and Drug Supervisory Agency of the Republic of Indonesia number HK. 00. 06. 1. 52. 401, the maximum limit of the Most Probable Number (MPN) Coliform in tomato sauce is 100 colonies/g.
References
Adelberg, Jawetz and Melnick. 2017. Mikrobiologi kedokteran Jawetz, Melnick, & Adelberg, Ed.23, Translation of Jawetz, Melnick, and Adelberg’s Medical Microbiology, 23thEd, EGC, Jakarta.
Amaliyah, N. (2017). Penyehatan Makanan dan Minuman. Yogyakarta : Deepublish.
Amelia, C.M. and Fayasari, A. (2020) “Faktor Yang Mempengaruhi Konsumsi Sayur Dan Buah Remaja di SMP Negeri 238 Jakarta,” Jurnal Gizi dan Pangan Soedirman, 4(1), hal. 94. Tersedia pada: http://jos.unsoed.ac.id/index.php/jg ps/article/view/2642/1474.
Amqam, H., Natsira, M.F., Thamrin, Y., Gunawan, N.A., Sari, Y.I., and Hermawati, E. 2021. Bacteria contamination analysis on the hands and bowls of meatball cart vendors: an observational descriptive study. Gac Sanit 35 (51), 571-575. https://doi.org/10.1016/j.gaceta.2020.12.020.
Amyati. 2020. Kontaminasi bakteri Eschericia coli pada makanan yang dijual di pasar tradisional Yogyakarta. Health Sciences and Pharmacy Journal Vol. 4, No. 2, Agustus 2020, pp. 54-59 2622-1268 (Print) http://journal.stikessuryaglobal.ac.id/index.php/hspj
Arini, L.D.D. 2016. Mitigasi Escherichia Coli Dalam Berbagai Makanan Di Pusat Jajanan Surakarta (Galabo). Jurnal Ekosains Vol 4 (2), 45-55, Https://Jurnal.Uns.Ac.Id/Ekosains/Article/View/62147/35909.
Arini, L.D.D., and Wulandari, R.M. 2017. Kontaminasi Bakteri Coliform Pada Saus Siomai Dari Pedagang Area Kampus Di Surakarta. Jurnal Biomedika Vol 10 (2) :31-46, Http://Ejurnal.Setiabudi.Ac.Id/Ojs/Index.Php/Biomedika/Article/View/273
Aulya, W., Fadhliani, F., and Mardina, V. (2022). Analysis of Coliform and Colifecal Total Pollution Test on Various Types of Drinking Water Using the MPN (Most Probable Number) Method. Serambi Journal of Agricultural Technology, Vol.2 No.2 Hal 64 - 72://ojs.serambimekkah.ac.id/index.php/sjat.
Budiarti, A. and Utami, M.P.P. (2021) “Konsumsi Makanan Cepat Saji pada Remaja di Surabaya,” Jurnal Ilmu Kesehatan MAKIA, 11(2), hal. 8–14. Tersedia pada: https://www.jurnal.stikesicsada.ac.i d/index.php/jmakia/article/view/167 /141.
Carlsen, M.H., Bjontegaard, M.M., Oort, B.V., Jafarzadeh, S., Metha, S., Wrigt, E.C., and Andersen, L.F. (2025). Environmental impact of Norwegian food and beverages: compilation of a life cycle assessment food database. Food & Nutrition Research, 69, page 1-7: 10645. http://dx.doi.org/10.29219/fnr.v69.10645.
Curticapean, M.C. (2022). Detection Of Escherichia Coli From Non-Sterile Herbal. Farmacia, Vol. 70, 2, 295-299. https://doi.org/10.31925/farmacia.2022.2.15.
Duan, K., Pang, G., Duan, Y., Onyeaka, H., and Krebs, J. (2025). Current research development on food contaminants, future risks, regulatory regime and detection technologies: A systematic literature review. Journal of Environmental Management Volume 381, 125246. https://doi.org/10.1016/j.jenvman.2025.125246.
Fatimah, S., Hekmah, N., Fathullah, D. M., and Norhasanah, N. (2022). Cemaran Mikrobiologi Pada Makanan, Alat Makan, Air Dan Kesehatan Penjamah Makanan Di Unit Instalasi Gizi Rumah Sakit X Di Banjarmasin. Journal of Nutrition College, 11(4), 322–327. https://doi.org/10.14710/jnc.v11i4.35300.
Gisslevi, E., Svanqvist, L., Skoog, I., and Rothenberg, E. (2025). Meal Pattern Variables and 15-Year Mortality: Results from the Gothenburg H70 Birth Cohort Studies, Sweden. Food & Nutrition Research, 69: 11445, page 1-12. http://dx.doi.org/10.29219/fnr.v69.11445.
Gupta, N., Jain, A., Sen, R., Mishra, S., and Gupta, M. 2022. A Study on Isolation of E. Coli Bacteria from Different Human Clinical Specimens. International Journal of Health Sciences and Research Vol.12; Issue: 6, page 255-263. https://doi.org/10.52403/ijhsr.20220633.
H, Ho, L., and S, Nurul, A. (2024). Physicochemical properties and sensory evaluation of gluten-free rice egg roll with hydrocolloids addition. Food Research 8 (1) : 257 – 266. https://doi.org/10.26656/fr.2017.8(1).272.
Hunowu, N.A., Nurfadillah, A.R., and Lalu, N.A.S. 2023. Analisis Risiko Bakteri Escherichia Coli Pada Makanan Di Pasar Jajan Kota. Prosiding Seminar Nasional Mini Riset Mahasiswa (e-ISSN : 2964-0202) Vol 2(1), Hal 90 – 98, https://ejurnal.ung.ac.id/index.php/semasetwa/article/view/21452/7197
Isnawaida,., Yuliati, F.N., Prahesti, K.I., Malaka, R., and Hajrawati. (2021). Detection of coliform bacteria, total plate count and pH value in chicken eggs from Maros traditional market. The 3rd International Conference of Animal Science and Technology IOP Conf. Series: Earth and Environmental Science 788 (2021) 012158 IOP Publishing page 1-3. doi:10.1088/1755-1315/788/1/012158.
Kalangi, B.J., Sanggelorang, Y., and Malonda, N.S.H. 2024. Keberagaman Konsumsi Makanan pada Remaja di Daerah Pesisir Kota Bitung Diversity of Food Consumption in Adolescents in the Coastal Area of Bitung City. Jurnal Dunia Kesmas, Vol. 13 (1) : 1-10, https://ejurnalmalahayati.ac.id/index.php/duniakesmas/article/view/10932.
Karlah, L. R. Mansauda; Fatimawali and Novel, Kojong. 2014. Analisis Cemaran Bakteri Coliform Pada Saus Tomat Jajanan Bakso Tusuk Yang Beredar Di Manado. Jurnal Ilmiah Farmasi. UNSRAT. Volume 3 no (2) hal 37-44.
Launde, A.P., Pioh, N.R., and Waworundeng, W. 2020. Tugas Dan Fungsi Badan Pengawas Obat Dan Makanan Dalam Melindungi Kesehatan Masyarakat Di Kota Manado (Studi Kasus Tentang Penggunaan Bahan Makanan Berbahaya Di Kota Manado). Eksekutif : Jurnal Ilmu Pemerintahan vol 4 (4):1-17, https://ejournal.unsrat.ac.id/v3/index.php/jurnaleksekutif/article/download/28626/27959/59014.
Lawati, H.A., Blair, B.M., and Larnard, J. (2024). Urinary Tract Infections: Core Curriculum. AJKD Vol 83 | Iss 1, page 90-100. https://doi.org/10.1053/j.ajkd.2023.08.009.
Lestari, D., Haliza, E.N., and Setiawan, H. (2024). Effect of pH Variations in Eosin Methylene Blue Agar (EMBA) Medium on E. coli Growth. Jurnal Info Kesehatan, 22(1), 82-89. https://doi.org/10.31965/infokes.Vol22Iss1.1298.
Li, H., Li L., Chi Y., Tian Q., Zhou T., and Han, C, … & Zhou Y, Development of a standardized Gram stain procedure for bacteria and inflammatory cells using an automated staining instrument. MicrobiologyOpen. 2020;9:e1099. https://doi.org/10.1002/mbo3.1099.
Manetu, W.M., M’masi, S., and Recha, C.W. (2021). Diarrhea Disease among Children under 5 Years of Age: A Global Systematic Review. Open Journal of Epidemiology, 2021, 11, 207-221. https://doi.org/10.4236/ojepi.2021.113018.
Nasution, A.S. 2020. Hygiene Penjamah Makanan Menyebabkan Kontaminasi Escherichia Coli Pada Jajanan Pasar Tradisional. Promotor Jurnal Mahasiswa Kesehatan Masyarakat Vol 3 (1) : 1-6. https://ejournal.uika-bogor.ac.id/index.php/PROMOTOR/article/view/3119.
Pakdel, M., Olsen, A., and Bar, E.M.S. (2023). A Review of Food Contaminants and Their Pathways Within Food Processing Facilities Using Open Food Processing Equipment. Journal of Food Protection Volume 86, Issue 12, 100184. https://doi.org/10.1016/j.jfp.2023.100184.
Pasek, I.N.S., Budiman., and Rismawati, N. 2020. Uji Kandungan Bakteri Escherichia Coli pada Makanan dan Kondisi Fasilitas Sanitasi Tempat Pengolahan Makanan di Bandara Mutiara Sis Al Jufri Palu. Jurnal Kolaboratif Sains Vol 3 (1) : 37-43, https://jurnal.unismuhpalu.ac.id/index.php/JKS/article/view/1685/1453.
Peraturan Badan Pengawas Obat Dan Makanan Nomor 13 Tahun 2019 Tentang Batas Maksimal Cemaran Mikroba Dalam Pangan Olahan.
Perez, M., Coria, J.G., Castellon, J.L., Valli, B.E., Yarena, A.L., Rodriguez, C.J., Pinto, D., Queralt, A.V., Bendini, A., and Raventos, M.L. (2024). Assessing the Impact of Cooking on Bioactive Compounds in Tomato Sauces with Added Tomato Pomace: A Chemometric Study. Research Square. https://doi.org/10.21203/rs.3.rs-4240753/v1.
Puspitasari, D., Noerhartati, E., and Mujianto. (2024). Characteristic of Egg Roll Product with Treatment Proportions of Kimpul-Mung Bean Composite Flour. Journal of Research and Technology volume 10 (1), 113-122.
Rahayu, W.P., Nurjanah, S., and Komalasari, E. 2018. Eschericia coli : Patogenitas, Analisis dan Kajian Resiko. Bogor : IPB Press. https://repository.uai.ac.id/wp-content/uploads/2020/09/B4_Buku.pdf.
Riyanto, A., and Abdillah, A. D. (2012). Faktor yang Memengaruhi Kandungan E. coli Makanan Jajanan SD di Wilayah Cimahi Selatan. Majalah Kedokteran Bandung, 44(2), 77–82. https://doi.org/10.15395/mkb.v44n2.127.
Sabaaturohma, C. L., Gelgel, K. T. P., and Suada, I. K. (2020). Jumlah Cemaran Bakteri Coliform dan Non-Coliform pada Air di RPU di Denpasar Melampaui Baku Mutu Nasional. Indonesia Medicus Veterinus, 9(1), 139–147. https://doi.org/10.19087/imv.2020.9.1.13 9.
Sihaloho, S.M., Suharmanto, T., Apriliana, E., and Pramesona, B.A. 2025. Faktor-Faktor Yang Berhubungan Dengan Keberadaan Escherichia Coli (E. Coli) Pada Makanan Dan Minuman. Journal of Language and Health Volume 6 (1) : 35-42, https://jurnal.globalhealthsciencegroup.com/index.php/JLH/article/view/5736/3935.
Siwi, V.H., and Mogea, A.R. 2022. Bakteri Escherichia coli pada Saus Kacang Jajanan Cilok di Kota Manado. Majalah InfoSains, 2022, 3(2) 90-94, https://www.jurnal.fmipaukit.ac.id/index.php/JIS/article/download/60/63.
Sugiah., Mutmaina, G.N., Mamay., and Nurisani, A. 2023. Isolation and identification of escherichia coli in well water located in Garut Regency. Science Midwifery vol 11 (1), page 195-201. www.midwifery.iocspublisher.org.
Suryani, D., and Astuti, F.D. 2019. Higiene dan Sanitasi pada Pedagang Angkringan di Kawasan Malioboro Yogyakarta. Jurnal Kedokteran dan Kesehatan, 15, 1, : 71.
Susanti, M., Pratama, A.R.P., Suryanti, M.I., Suryati and Dachriyanus. (2022). Development and validation of TLC-densitometry method for quantification of tetraprenyltoluquinone in the stem bark hexane extract of Garcinia cowa roxb. Heliyon Volume 8, Issue 9, September 2022, e10437. https://doi.org/10.1016/j.heliyon.2022.e10437.
Syamsussabri, M., Ningtyas, R.N., Najah, A.A., Fahmi, M.S., and Suarsini, E. (2018). Analysis of Coliform Bacteria Contamination in Drinking Water Sources in Malang City. El-Hayah Vol. 7, No.1 (2018) 28-35. http://ejournal.uin-malang.ac.id/index.php/bio/index.
Walid, D.L., Afiah, A.S.N., and Rahman, I. 2021. Identifikasi Escherichia Coli Pada Makanan Di Rumah Makan Di Lingkungan Kampus Ii Universitas Khairun. Kieraha Medical Journal vol 3 (1), 57-65, https://ejournal.unkhair.ac.id/index.php/kmj/article/view/3271.
Wardani, D.L., and Setianingrum, Z. 2019. Identifikasi Bakteri Escherichia coli Pada Saus Makanan Jajanan Di Sekitar Kampus Universitas Muhammadiyah Surakarta. . Jurnal Kesehatan Vol. 12 (2) 2019, 91-101. https://journals.ums.ac.id/index.php/jk/article/view/9765/5090.
