ANTIHYPERURESEMIA ACTIVITY OF ETHANOL EXTRACTS AND FRACTIONS OF NIPAH LEAF FRONDS (NYPA FRUTICANS. WURMB)
DOI:
https://doi.org/10.47701/icohetech.v4i1.3397Keywords:
Nypa fruticans, Wurmb, Xanthine oxidase, Antihyperuresaemia, FractionsAbstract
Hyperuresemia is a metabolite syndrome characterised by high levels of uric acid in the blood. Hyperuresmia in advanced stages can cause gout or pyrexia (a disease that affects the joints and causes joint damage), kidney stones, kidney damage, and hypertension. The nipah plant (Nypa fruticans. Wurmb) is traditionally used in the treatment of stomach pain, diabetes, fever, toothache, uric acid reduction and headache medicine. In general, the content of secondary metabolite compounds from nipah plants includes flavonoids, phenols, tannins, terpenois, steroids, alkaloids and saponins. The purpose of this study was to determine the xanthine oxidase inhibitory activity of ethanol extracts and fractions of nipah leaves (Nypa fruticans. Wurmb). Extraction process using maceration method with 96% ethanol solvent, fractionation using liquid-liquid partition method with separating funnel. . Testing the inhibition of xanthine oxidase enzyme activity in vitro using UV/VIS spectrophotometer. The results showed that ethanol extract, water fraction, ethyl acetate fraction and n-hexane fraction showed inhibitory activity of xanthine oxidase enzyme with IC50 values of 47.558 ± 2.862; 18.755 ± 3.821; 3.543 ± 1.440 and 110.213 ± 1.577 ppm, respectively. From the results of the study it can be concluded that ethanol extracts and fractions of nipah leaf fronds (Nypa fruticans.Wurmb) can inhibit the activity of xanthine oxidase enzyme with ethyl acetate fraction giving the highest inhibitory activity with IC50 value of 3,543±1,440 ppm.
References
Astuti, Maria Dewi, Nisa, Khairatun, & Mustikasari, Kamilia. (2020). Identification of Chemical Compounds from Nipah (Nypa fruticans Wurmb.) Endosperm. BIO Web of Conferences, 20, 3002. EDP Sciences.
Badri, Putri Rizki Amalia, Rosita, Yanti, & Peratiwi, Della. (2020). Analisis Faktor-Faktor Yang Mempengaruhi Pengetahuan Masyarakat Tentang Faktor Risiko Hiperurisemia. Syifa’Med J Kedokt Dan Kesehat, 10(2).
Djakad, Siti Rosida R. (2020). Inhibisi Enzim Xantin Oksidase dari Susu Sapi Dengan Ekstrak Daun Kelor (Moringa olivera) Dan Daun Jeruk Nipis (Citrus Aurantifolia). Universitas Hasanuddin.
Ghanbari, Mohammad Hossein, Mashhadizadeh, Mohammad Hossein, Norouzi, Zahra, & Salehzadeh, Hamid. (2022). Simultaneous Electrochemical Detection Of Uric Acid And Xanthine Based On Electrodeposited B, N Co-Doped Reduced Graphene Oxide, Gold Nanoparticles And Electropolymerized Poly (L-Cysteine) Gradually Modified Electrode Platform. Microchemical Journal, 175, 107213.
Harborne, Jeffrey Barry. (1987). Metode fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. Bandung: Penerbit ITB, 78.
Havlik, J., Rada, V., & Plachy, V. (2011). Xanthine Oxidase-Inhibitory And Hypouricemic Action Of Black Poplar Bud Extract. Planta Medica, 77(12), PM213.
Julianan, F. (2022). Pengaruh Pemberian Sirup Ekstrak Daun Nipah (Nypa fruticans wurmb) Pada Tikus Putih Jantan Sebagai Penurun Kadar Asam Urat. Universitas Al-Irsyad Cilacap.
Juwita, Retno, Saleh, Chairul, & Sitorus, Saibun. (2017). Uji Aktivitas Antihiperurisemia Dari Daun Hijau Tanaman Pucuk Merah (Syzygium myrtifolium Walp.) Terhadap Mencit Jantan (Mus musculus). Jurnal Atomik, 2(1), 162–168.
Khairi, Ikhsanul, Bahri, Samsul, Ukhty, Nabila, Rozi, Anhar, & Nasution, Muhammad Arif. (2020). Potensi Pemanfaatan Nipah (Nypa Fruticans) sebagai Pangan Fungsional dan Farmasetika. Jurnal Laot Ilmu Kelautan, 2(2), 119–128.
Liu, Ning, Xu, Hu, Sun, Qianqian, Yu, Xiaojuan, Chen, Wentong, Wei, Hongquan, Jiang, Jie, Xu, Youzhi, & Lu, Wenjie. (2021). The Role Of Oxidative Stress In Hyperuricemia And Xanthine Oxidoreductase (XOR) Inhibitors. Oxidative Medicine and Cellular Longevity, 2021.
Malik, Neelam, Dhiman, Priyanka, Sobarzo-Sanchez, Eduardo, & Khatkar, Anurag. (2018). Flavonoids And Anthranquinones As Xanthine Oxidase And Monoamine Oxidase Inhibitors: A New Approach Towards Inflammation and Oxidative Stress. Current Topics in Medicinal Chemistry, 18(25), 2154–2164.
Moonrungsee, Nuntaporn, Jakmunee, Jaroon, Peamaroon, Nipat, Boonmee, Apaporn, Kasemsuk, Teerapich, Seeda, Sureerat, & Suwancharoen, Sunisa. (2022). Phytochemical and Xanthine Oxidase Inhibitory Activity in Nypa fruticans Wurmb. Fruit Extracts. Trends in Sciences, 19(4), 2583.
Organization, World Health. (2013). WHO traditional medicine strategy: 2014-2023. World Health Organization.
Prasad, Nagendra, Yang, Bao, Kong, Kin Weng, Khoo, Hock Eng, Sun, Jian, Azlan, Azrina, Ismail, Amin, & Romli, Zulfiki Bin. (2013). Phytochemicals and Antioxidant Capacity From Nypa fruticans Wurmb. Fruit. Evidence-Based Complementary and Alternative Medicine, 2013.
Raharjo, Danang. (2022). Efektivitas Penghambatan Enzim Xantin Oksidase Ekstrak Etanol Dan Fraksi Etanol, Fraks Etil Asetat Serta Fraksi N-Heksane Kulit Batang Mangrove Merah (Rhizopora Mucronata). Jurnal Ilmiah Kesehatan, 15(1), 63–70.
Raharjo, Danang, & Pambudi, Dwi Bagus. (2021). Efek Anti Hiperuresemia Ekstrak Etanol Dan Fraksi Kulit Buah Pepaya (Carica papaya L). Prosiding Seminar Informasi Kesehatan Nasional, 61–66.
Sithara, N. V, Komathi, S., Rajalakshmi, G., Queen, J., & Bharathi, D. (2016). Phytochemical Analysis of Andrographis paniculata Using Different Solvents. European Journal of Biotechnology and Bioscience, 4(8), 28–30.
Tripolino, Cesare, Ciaffi, Jacopo, Ruscitti, Piero, Giacomelli, Roberto, Meliconi, Riccardo, & Ursini, Francesco. (2021). Hyperuricemia in psoriatic arthritis: epidemiology, pathophysiology, and clinical implications. Frontiers in Medicine, 8, 737573.
Widuri, Anggita, Mun’im, Abdul, & Katrin, Katrin. (2016). Uji Aktivitas Penghambatan Xantin Oksidase Ekstrak Etanol 80% Dari Tanaman Famili Combretaceae, Lauraceae, Lythraceae, Oxalidaceae, Piperaceae, Plumbaginaceae, dan Smilacaceae. FITOFARMAKA: Jurnal Ilmiah Farmasi, 6(2), 77–87.
Xue, Hui, Xu, Meng, Gong, Deming, & Zhang, Guowen. (2023). Mechanism of Flavonoids Inhibiting Xanthine Oxidase And Alleviating Hyperuricemia From Structure–Activity Relationship And Animal Experiments: A review. Food Frontiers.
Zhao, Jie, Huang, Lin, Sun, Chunyong, Zhao, Dongsheng, & Tang, Hongjin. (2020). Studies on The Structure-Activity Relationship and Interaction Mechanism of Flavonoids and Xanthine Oxidase Through Enzyme Kinetics, Spectroscopy Methods and Molecular Simulations. Food Chemistry, 323, 126807.
